Key: Win : Draw : Loss
| Date | Home | Away | Ref | ||
|---|---|---|---|---|---|
| 08/08/2009 | Gillingham | 5 | 0 | Swindon | G Horwood |
| 15/08/2009 | Swindon | 0 | 0 | Milton Keynes Dons | G Ward |
| 18/08/2009 | Swindon | 1 | 0 | Southampton | S Cook |
| 22/08/2009 | Oldham | 2 | 2 | Swindon | M Haywood |
| 29/08/2009 | Swindon | 2 | 1 | Southend | M Russell |
| 05/09/2009 | Yeovil | 0 | 1 | Swindon | J Singh |
| 12/09/2009 | Swindon | 1 | 1 | Colchester | K Evans |
| 19/09/2009 | Walsall | 1 | 1 | Swindon | D Webb |
| 26/09/2009 | Swindon | 1 | 1 | Wycombe | A Hall |
| 29/09/2009 | Exeter | 1 | 1 | Swindon | K Stroud |
| 03/10/2009 | Brentford | 2 | 3 | Swindon | O Langford |
| 10/10/2009 | Swindon | 1 | 1 | Millwall | K Wright |
| 17/10/2009 | Swindon | 0 | 2 | Hartlepool | T Kettle |
| 24/10/2009 | Norwich | 1 | 0 | Swindon | G Hegley |
| 31/10/2009 | Tranmere | 1 | 4 | Swindon | A Bates |
| 21/11/2009 | Carlisle | 0 | 1 | Swindon | C Webster |
| 24/11/2009 | Swindon | 2 | 1 | Huddersfield | A Penn |
| 05/12/2009 | Swindon | 3 | 2 | Leyton Orient | G Sutton |
| 12/12/2009 | Bristol Rvs | 3 | 0 | Swindon | P Walton |
| 19/12/2009 | Swindon | 2 | 1 | Brighton | J Moss |
| 26/12/2009 | Charlton | 2 | 2 | Swindon | P Miller |
| 28/12/2009 | Swindon | 3 | 1 | Yeovil | K Stroud |
| 16/01/2010 | Swindon | 3 | 1 | Gillingham | S Tanner |
| 19/01/2010 | Stockport | 0 | 1 | Swindon | G Salisbury |
| 26/01/2010 | Swindon | 3 | 0 | Leeds | P Gibbs |
| 30/01/2010 | Southend | 2 | 2 | Swindon | D Phillips |
| 06/02/2010 | Swindon | 1 | 1 | Charlton | A Hall |
| 09/02/2010 | Swindon | 4 | 2 | Oldham | D Deadman |
| 13/02/2010 | Huddersfield | 2 | 2 | Swindon | S Rushton |
| 20/02/2010 | Swindon | 2 | 0 | Carlisle | D Sheldrake |
| 23/02/2010 | Swindon | 4 | 1 | Stockport | R East |
| 27/02/2010 | Leyton Orient | 0 | 0 | Swindon | C Pawson |
| 06/03/2010 | Swindon | 0 | 4 | Bristol Rvs | P Taylor |
| 09/03/2010 | Milton Keynes Dons | 2 | 1 | Swindon | J Linnington |
| 13/03/2010 | Brighton | 0 | 1 | Swindon | G Hegley |
| 16/03/2010 | Southampton | 0 | 1 | Swindon | K Hill |
| 20/03/2010 | Swindon | 1 | 1 | Norwich | S Mathieson |
| 27/03/2010 | Hartlepool | 0 | 1 | Swindon | M Oliver |
| 03/04/2010 | Leeds | 0 | 3 | Swindon | M Russell |
| 05/04/2010 | Swindon | 3 | 0 | Tranmere | O Langford |
| 10/04/2010 | Colchester | 3 | 0 | Swindon | A Penn |
| 12/04/2010 | Swindon | 1 | 1 | Exeter | N Swarbrick |
| 17/04/2010 | Swindon | 1 | 1 | Walsall | S Cook |
| 24/04/2010 | Wycombe | 2 | 2 | Swindon | S Attwell |
| 01/05/2010 | Swindon | 3 | 2 | Brentford | A Woolmer |
| 08/05/2010 | Millwall | 3 | 2 | Swindon | K Stroud |