Key: Win : Draw : Loss
| Date | Home | Away | Ref | ||
|---|---|---|---|---|---|
| 08/08/2009 | Crystal Palace | 1 | 1 | Plymouth | A Penn |
| 15/08/2009 | Plymouth | 1 | 1 | QPR | R East |
| 18/08/2009 | Plymouth | 1 | 3 | Cardiff | R Booth |
| 22/08/2009 | Derby | 2 | 1 | Plymouth | C Pawson |
| 29/08/2009 | Plymouth | 1 | 3 | Sheffield Weds | A Haines |
| 12/09/2009 | West Brom | 3 | 1 | Plymouth | G Salisbury |
| 15/09/2009 | Plymouth | 0 | 1 | Watford | G Scott |
| 19/09/2009 | Newcastle | 3 | 1 | Plymouth | S Mathieson |
| 27/09/2009 | Plymouth | 0 | 1 | Nott'm Forest | A Woolmer |
| 29/09/2009 | Peterboro | 1 | 2 | Plymouth | A Hall |
| 03/10/2009 | Plymouth | 2 | 1 | Scunthorpe | A D'Urso |
| 17/10/2009 | Blackpool | 2 | 0 | Plymouth | D Webb |
| 20/10/2009 | Bristol City | 3 | 1 | Plymouth | G Hegley |
| 24/10/2009 | Plymouth | 1 | 1 | Ipswich | S Hooper |
| 31/10/2009 | Middlesbrough | 0 | 1 | Plymouth | M Russell |
| 07/11/2009 | Plymouth | 2 | 1 | Doncaster | P Taylor |
| 21/11/2009 | Leicester | 1 | 0 | Plymouth | D McDermid |
| 05/12/2009 | Plymouth | 0 | 1 | Sheffield United | G Horwood |
| 08/12/2009 | Swansea | 1 | 0 | Plymouth | S Tanner |
| 12/12/2009 | Preston | 2 | 0 | Plymouth | M Oliver |
| 19/12/2009 | Plymouth | 0 | 1 | Coventry | K Stroud |
| 26/12/2009 | Cardiff | 0 | 1 | Plymouth | P Gibbs |
| 28/12/2009 | Plymouth | 4 | 1 | Reading | S Hooper |
| 16/01/2010 | Plymouth | 0 | 1 | Crystal Palace | A Bates |
| 26/01/2010 | Plymouth | 1 | 0 | Derby | A Hall |
| 30/01/2010 | Sheffield Weds | 2 | 1 | Plymouth | K Wright |
| 06/02/2010 | Plymouth | 0 | 1 | West Brom | S Tanner |
| 09/02/2010 | Reading | 2 | 1 | Plymouth | O Langford |
| 13/02/2010 | Barnsley | 1 | 3 | Plymouth | K Evans |
| 16/02/2010 | Plymouth | 1 | 1 | Swansea | K Hill |
| 20/02/2010 | Plymouth | 1 | 1 | Leicester | R East |
| 27/02/2010 | Sheffield United | 4 | 3 | Plymouth | C Sarginson |
| 06/03/2010 | Plymouth | 1 | 1 | Preston | A Woolmer |
| 09/03/2010 | QPR | 2 | 0 | Plymouth | P Tierney |
| 13/03/2010 | Coventry | 1 | 1 | Plymouth | A Haines |
| 16/03/2010 | Plymouth | 3 | 2 | Bristol City | T Kettle |
| 20/03/2010 | Scunthorpe | 2 | 1 | Plymouth | A Taylor |
| 23/03/2010 | Ipswich | 0 | 2 | Plymouth | D Whitestone |
| 27/03/2010 | Plymouth | 0 | 2 | Blackpool | J Linington |
| 30/03/2010 | Plymouth | 0 | 0 | Barnsley | M Russell |
| 03/04/2010 | Doncaster | 1 | 2 | Plymouth | K Wright |
| 05/04/2010 | Plymouth | 0 | 2 | Middlesbrough | S Mathieson |
| 10/04/2010 | Watford | 1 | 0 | Plymouth | W Atkins |
| 19/04/2010 | Plymouth | 0 | 2 | Newcastle | C Pawson |
| 24/04/2010 | Nott'm Forest | 3 | 0 | Plymouth | N Swarbrick |
| 02/05/2010 | Plymouth | 1 | 2 | Peterboro | S Tanner |