Key: Win : Draw : Loss
| Date | Home | Away | Ref | ||
|---|---|---|---|---|---|
| 08/08/2009 | Southampton | 1 | 1 | Millwall | M Russell |
| 15/08/2009 | Millwall | 0 | 0 | Carlisle | D Deadman |
| 18/08/2009 | Millwall | 2 | 0 | Oldham | A D'Urso |
| 21/08/2009 | Southend | 0 | 0 | Millwall | D Whitestone |
| 28/08/2009 | Millwall | 1 | 1 | Brighton | A Penn |
| 05/09/2009 | Bristol Rvs | 2 | 0 | Millwall | J Moss |
| 12/09/2009 | Gillingham | 2 | 0 | Millwall | K Stroud |
| 19/09/2009 | Millwall | 3 | 1 | Huddersfield | P Crossley |
| 26/09/2009 | Leyton Orient | 1 | 0 | Millwall | S Tanner |
| 29/09/2009 | Millwall | 0 | 0 | Yeovil | S Cook |
| 03/10/2009 | Millwall | 5 | 0 | Tranmere | G Scott |
| 10/10/2009 | Swindon | 1 | 1 | Millwall | K Wright |
| 17/10/2009 | Stockport | 0 | 4 | Millwall | M Haywood |
| 24/10/2009 | Millwall | 2 | 1 | Leeds | M Clattenburg |
| 31/10/2009 | Millwall | 2 | 1 | Colchester | G Horwood |
| 14/11/2009 | Brentford | 2 | 2 | Millwall | A Woolmer |
| 21/11/2009 | Millwall | 0 | 2 | Wycombe | R Booth |
| 24/11/2009 | Exeter | 1 | 1 | Millwall | S Hooper |
| 01/12/2009 | Millwall | 3 | 2 | Milton Keynes Dons | R East |
| 05/12/2009 | Hartlepool | 3 | 0 | Millwall | J Waugh |
| 12/12/2009 | Millwall | 2 | 1 | Walsall | A D'Urso |
| 19/12/2009 | Charlton | 4 | 4 | Millwall | M Jones |
| 26/12/2009 | Norwich | 2 | 0 | Millwall | G Salisbury |
| 28/12/2009 | Millwall | 2 | 0 | Bristol Rvs | F Graham |
| 16/01/2010 | Millwall | 1 | 1 | Southampton | A Woolmer |
| 23/01/2010 | Oldham | 0 | 1 | Millwall | A Haines |
| 26/01/2010 | Millwall | 2 | 0 | Southend | K Evans |
| 30/01/2010 | Brighton | 0 | 1 | Millwall | K Friend |
| 06/02/2010 | Millwall | 2 | 1 | Norwich | G Hegley |
| 13/02/2010 | Millwall | 1 | 0 | Exeter | P Gibbs |
| 20/02/2010 | Wycombe | 1 | 0 | Millwall | A Hall |
| 23/02/2010 | Milton Keynes Dons | 1 | 3 | Millwall | D Whitestone |
| 27/02/2010 | Millwall | 1 | 0 | Hartlepool | M Russell |
| 06/03/2010 | Walsall | 2 | 2 | Millwall | K Hill |
| 09/03/2010 | Carlisle | 1 | 3 | Millwall | G Salisbury |
| 13/03/2010 | Millwall | 4 | 0 | Charlton | N Swarbrick |
| 22/03/2010 | Leeds | 0 | 2 | Millwall | C Foy |
| 27/03/2010 | Millwall | 5 | 0 | Stockport | P Miller |
| 02/04/2010 | Millwall | 1 | 1 | Brentford | P Taylor |
| 05/04/2010 | Colchester | 1 | 2 | Millwall | A D'Urso |
| 10/04/2010 | Millwall | 4 | 0 | Gillingham | M Haywood |
| 13/04/2010 | Yeovil | 1 | 1 | Millwall | M Russell |
| 16/04/2010 | Huddersfield | 1 | 0 | Millwall | J Linnington |
| 24/04/2010 | Millwall | 2 | 1 | Leyton Orient | R East |
| 01/05/2010 | Tranmere | 2 | 0 | Millwall | N Swarbrick |
| 08/05/2010 | Millwall | 3 | 2 | Swindon | K Stroud |