Key: Win : Draw : Loss
| Date | Home | Away | Ref | ||
|---|---|---|---|---|---|
| 06/08/2016 | Cambridge | 1 | 1 | Barnet | G Horwood |
| 13/08/2016 | Colchester | 2 | 0 | Cambridge | O Yates |
| 16/08/2016 | Doncaster | 1 | 0 | Cambridge | D Handley |
| 20/08/2016 | Cambridge | 2 | 2 | Carlisle | B Huxtable |
| 27/08/2016 | Cambridge | 0 | 3 | Luton | D Webb |
| 03/09/2016 | Mansfield | 0 | 0 | Cambridge | D Whitestone |
| 10/09/2016 | Plymouth | 2 | 1 | Cambridge | L Probert |
| 17/09/2016 | Cambridge | 1 | 2 | Morecambe | D England |
| 24/09/2016 | Newport County | 1 | 2 | Cambridge | N Kinseley |
| 27/09/2016 | Cambridge | 1 | 0 | Yeovil | M Haywood |
| 01/10/2016 | Cambridge | 2 | 1 | Accrington | O Yates |
| 08/10/2016 | Blackpool | 1 | 1 | Cambridge | R Clark |
| 15/10/2016 | Cambridge | 0 | 1 | Grimsby | C Breakspear |
| 22/10/2016 | Exeter | 1 | 2 | Cambridge | R Jones |
| 29/10/2016 | Cambridge | 0 | 1 | Portsmouth | R Joyce |
| 12/11/2016 | Crawley Town | 1 | 3 | Cambridge | J Busby |
| 19/11/2016 | Cambridge | 1 | 2 | Wycombe | T Kettle |
| 22/11/2016 | Notts County | 0 | 1 | Cambridge | D Webb |
| 26/11/2016 | Cambridge | 3 | 1 | Cheltenham | R Joyce |
| 10/12/2016 | Hartlepool | 0 | 5 | Cambridge | C Boyeson |
| 17/12/2016 | Cambridge | 2 | 1 | Crewe | L Collins |
| 26/12/2016 | Stevenage | 1 | 2 | Cambridge | D Whitestone |
| 31/12/2016 | Leyton Orient | 1 | 1 | Cambridge | L Swabey |
| 02/01/2017 | Cambridge | 4 | 0 | Notts County | B Huxtable |
| 14/01/2017 | Cambridge | 0 | 0 | Blackpool | D Drysdale |
| 21/01/2017 | Cambridge | 1 | 3 | Mansfield | G Ward |
| 28/01/2017 | Luton | 2 | 0 | Cambridge | S Stockbridge |
| 04/02/2017 | Cambridge | 0 | 1 | Plymouth | J Brooks |
| 11/02/2017 | Morecambe | 2 | 0 | Cambridge | N Miller |
| 14/02/2017 | Yeovil | 1 | 1 | Cambridge | B Huxtable |
| 18/02/2017 | Cambridge | 3 | 2 | Newport County | M Heywood |
| 25/02/2017 | Barnet | 0 | 1 | Cambridge | C Breakspear |
| 28/02/2017 | Cambridge | 2 | 3 | Doncaster | R Joyce |
| 04/03/2017 | Cambridge | 1 | 1 | Colchester | E Ilderton |
| 11/03/2017 | Carlisle | 0 | 3 | Cambridge | R Jones |
| 14/03/2017 | Cambridge | 0 | 1 | Hartlepool | D Whitestone |
| 18/03/2017 | Cheltenham | 0 | 1 | Cambridge | G Salisbury |
| 25/03/2017 | Cambridge | 0 | 0 | Stevenage | M Haywood |
| 28/03/2017 | Accrington | 2 | 0 | Cambridge | J Busby |
| 01/04/2017 | Crewe | 1 | 2 | Cambridge | S Stockbridge |
| 08/04/2017 | Cambridge | 3 | 0 | Leyton Orient | N Kinseley |
| 14/04/2017 | Grimsby | 2 | 1 | Cambridge | A Haines |
| 17/04/2017 | Cambridge | 1 | 0 | Exeter | D Deadman |
| 22/04/2017 | Portsmouth | 2 | 1 | Cambridge | G Ward |
| 29/04/2017 | Cambridge | 2 | 0 | Crawley Town | B Huxtable |
| 06/05/2017 | Wycombe | 1 | 0 | Cambridge | M Heywood |