Key: Win : Draw : Loss
| Date | Home | Away | Ref | ||
|---|---|---|---|---|---|
| 08/08/2009 | Brighton | 0 | 1 | Walsall | J Singh |
| 15/08/2009 | Brentford | 0 | 0 | Brighton | T Kettle |
| 18/08/2009 | Huddersfield | 7 | 1 | Brighton | N Swarbrick |
| 22/08/2009 | Brighton | 2 | 4 | Stockport | S Cook |
| 28/08/2009 | Millwall | 1 | 1 | Brighton | A Penn |
| 05/09/2009 | Brighton | 1 | 0 | Wycombe | G Ward |
| 12/09/2009 | Carlisle | 0 | 2 | Brighton | A Haines |
| 19/09/2009 | Brighton | 2 | 3 | Southend | R East |
| 26/09/2009 | Bristol Rvs | 1 | 1 | Brighton | F Graham |
| 03/10/2009 | Brighton | 0 | 1 | Milton Keynes Dons | P Taylor |
| 10/10/2009 | Yeovil | 2 | 2 | Brighton | O Langford |
| 13/10/2009 | Brighton | 2 | 0 | Gillingham | G Scott |
| 17/10/2009 | Tranmere | 2 | 1 | Brighton | J Waugh |
| 24/10/2009 | Brighton | 0 | 2 | Oldham | D Whitestone |
| 31/10/2009 | Brighton | 3 | 3 | Hartlepool | P Miller |
| 15/11/2009 | Southampton | 1 | 3 | Brighton | K Friend |
| 21/11/2009 | Brighton | 0 | 3 | Leeds | J Linington |
| 24/11/2009 | Norwich | 4 | 1 | Brighton | K Hill |
| 01/12/2009 | Brighton | 0 | 2 | Charlton | G Ward |
| 05/12/2009 | Exeter | 0 | 1 | Brighton | R Shoebridge |
| 11/12/2009 | Brighton | 1 | 2 | Colchester | S Tanner |
| 19/12/2009 | Swindon | 2 | 1 | Brighton | J Moss |
| 26/12/2009 | Brighton | 0 | 0 | Leyton Orient | P Crossley |
| 28/12/2009 | Wycombe | 2 | 5 | Brighton | S Rushton |
| 16/01/2010 | Walsall | 1 | 2 | Brighton | G Horwood |
| 26/01/2010 | Stockport | 1 | 1 | Brighton | P Quinn |
| 30/01/2010 | Brighton | 0 | 1 | Millwall | K Friend |
| 06/02/2010 | Leyton Orient | 1 | 1 | Brighton | S Cook |
| 09/02/2010 | Brighton | 0 | 0 | Huddersfield | S Bratt |
| 13/02/2010 | Brighton | 1 | 2 | Norwich | D Whitestone |
| 20/02/2010 | Leeds | 1 | 1 | Brighton | C Boyeson |
| 23/02/2010 | Charlton | 1 | 2 | Brighton | A D'Urso |
| 27/02/2010 | Brighton | 2 | 0 | Exeter | S Rushton |
| 08/03/2010 | Colchester | 0 | 0 | Brighton | A Bates |
| 13/03/2010 | Brighton | 0 | 1 | Swindon | G Hegley |
| 16/03/2010 | Brighton | 3 | 0 | Brentford | D McDermid |
| 20/03/2010 | Oldham | 0 | 2 | Brighton | D Webb |
| 27/03/2010 | Brighton | 3 | 0 | Tranmere | P Taylor |
| 01/04/2010 | Brighton | 2 | 2 | Southampton | S Tanner |
| 05/04/2010 | Hartlepool | 2 | 0 | Brighton | M Haywood |
| 10/04/2010 | Brighton | 1 | 2 | Carlisle | K Wright |
| 13/04/2010 | Gillingham | 1 | 1 | Brighton | J Linington |
| 17/04/2010 | Southend | 0 | 1 | Brighton | S Hooper |
| 24/04/2010 | Brighton | 2 | 1 | Bristol Rvs | C Sarginson |
| 01/05/2010 | Milton Keynes Dons | 0 | 0 | Brighton | O Langford |
| 08/05/2010 | Brighton | 1 | 0 | Yeovil | S Tanner |