Key: Win : Draw : Loss
| Date | Home | Away | Ref | ||
|---|---|---|---|---|---|
| 04/08/2018 | Stevenage | 2 | 2 | Tranmere | C Breakspear |
| 11/08/2018 | Tranmere | 1 | 0 | Cheltenham | B Toner |
| 18/08/2018 | Swindon | 3 | 2 | Tranmere | A Young |
| 21/08/2018 | Tranmere | 0 | 0 | Mansfield | T Bramall |
| 25/08/2018 | Tranmere | 1 | 0 | Port Vale | D Whitestone |
| 01/09/2018 | Northampton | 1 | 1 | Tranmere | N Kinseley |
| 08/09/2018 | Tranmere | 1 | 1 | Colchester | R Lewis |
| 15/09/2018 | Carlisle | 0 | 2 | Tranmere | M Donohue |
| 22/09/2018 | Tranmere | 0 | 1 | Newport County | C Boyeson |
| 29/09/2018 | Milton Keynes Dons | 1 | 1 | Tranmere | L Collins |
| 02/10/2018 | Tranmere | 1 | 0 | Lincoln | A Haines |
| 06/10/2018 | Morecambe | 3 | 4 | Tranmere | T Kettle |
| 12/10/2018 | Tranmere | 1 | 0 | Macclesfield | A Backhouse |
| 20/10/2018 | Yeovil | 0 | 0 | Tranmere | C Sarginson |
| 23/10/2018 | Forest Green | 3 | 1 | Tranmere | A Coggins |
| 27/10/2018 | Tranmere | 5 | 1 | Crawley Town | T Nield |
| 03/11/2018 | Tranmere | 2 | 0 | Exeter | T Bramall |
| 17/11/2018 | Crewe | 3 | 2 | Tranmere | C Boyeson |
| 24/11/2018 | Tranmere | 1 | 1 | Oldham | S Stockbridge |
| 27/11/2018 | Grimsby | 5 | 2 | Tranmere | D Drysdale |
| 08/12/2018 | Tranmere | 1 | 0 | Cambridge | O Yates |
| 15/12/2018 | Notts County | 3 | 2 | Tranmere | R Lewis |
| 22/12/2018 | Bury | 2 | 1 | Tranmere | A Haines |
| 26/12/2018 | Tranmere | 3 | 1 | Morecambe | S Oldham |
| 29/12/2018 | Tranmere | 0 | 0 | Yeovil | C Breakspear |
| 01/01/2019 | Macclesfield | 1 | 1 | Tranmere | M Salisbury |
| 12/01/2019 | Cheltenham | 1 | 3 | Tranmere | A Coggins |
| 19/01/2019 | Tranmere | 1 | 2 | Swindon | R Joyce |
| 26/01/2019 | Mansfield | 3 | 0 | Tranmere | B Toner |
| 05/02/2019 | Tranmere | 1 | 2 | Northampton | P Marsden |
| 09/02/2019 | Tranmere | 2 | 0 | Stevenage | E Ilderton |
| 16/02/2019 | Cambridge | 0 | 0 | Tranmere | B Huxtable |
| 19/02/2019 | Port Vale | 1 | 2 | Tranmere | C Hicks |
| 23/02/2019 | Tranmere | 1 | 0 | Notts County | S Oldham |
| 02/03/2019 | Exeter | 0 | 1 | Tranmere | J Busby |
| 08/03/2019 | Tranmere | 1 | 0 | Crewe | M Coy |
| 12/03/2019 | Tranmere | 4 | 1 | Grimsby | G Salisbury |
| 23/03/2019 | Colchester | 0 | 2 | Tranmere | R Joyce |
| 30/03/2019 | Tranmere | 3 | 0 | Carlisle | S Duncan |
| 02/04/2019 | Oldham | 2 | 0 | Tranmere | M Donohue |
| 06/04/2019 | Newport County | 0 | 0 | Tranmere | A Young |
| 13/04/2019 | Tranmere | 2 | 1 | Milton Keynes Dons | J Brooks |
| 19/04/2019 | Tranmere | 0 | 1 | Forest Green | D Whitestone |
| 22/04/2019 | Lincoln | 0 | 0 | Tranmere | A Backhouse |
| 30/04/2019 | Tranmere | 1 | 1 | Bury | D Webb |
| 04/05/2019 | Crawley Town | 3 | 1 | Tranmere | A Haines |