Key: Win : Draw : Loss
| Date | Home | Away | Ref | ||
|---|---|---|---|---|---|
| 05/08/2017 | Accrington | 3 | 1 | Colchester | R Joyce |
| 12/08/2017 | Colchester | 1 | 1 | Stevenage | A Coggins |
| 19/08/2017 | Luton | 3 | 0 | Colchester | S Stockbridge |
| 26/08/2017 | Colchester | 5 | 1 | Forest Green | C Hicks |
| 02/09/2017 | Cambridge | 1 | 0 | Colchester | N Kinseley |
| 09/09/2017 | Colchester | 3 | 1 | Crawley Town | G Horwood |
| 12/09/2017 | Colchester | 1 | 1 | Chesterfield | D Handley |
| 16/09/2017 | Cheltenham | 3 | 1 | Colchester | T Nield |
| 23/09/2017 | Colchester | 1 | 2 | Wycombe | B Huxtable |
| 26/09/2017 | Grimsby | 2 | 2 | Colchester | D Drysdale |
| 30/09/2017 | Yeovil | 0 | 1 | Colchester | A Coggins |
| 07/10/2017 | Colchester | 2 | 0 | Mansfield | J Busby |
| 14/10/2017 | Colchester | 0 | 1 | Carlisle | C Breakspear |
| 17/10/2017 | Newport County | 1 | 2 | Colchester | D England |
| 21/10/2017 | Coventry | 0 | 0 | Colchester | M Salisbury |
| 28/10/2017 | Colchester | 3 | 1 | Crewe | K Johnson |
| 11/11/2017 | Barnet | 0 | 1 | Colchester | J Brooks |
| 18/11/2017 | Colchester | 0 | 0 | Morecambe | L Collins |
| 21/11/2017 | Colchester | 1 | 0 | Lincoln | G Horwood |
| 25/11/2017 | Notts County | 2 | 1 | Colchester | M Heywood |
| 09/12/2017 | Colchester | 3 | 1 | Exeter | D Drysdale |
| 15/12/2017 | Swindon | 2 | 3 | Colchester | J Busby |
| 23/12/2017 | Colchester | 1 | 1 | Port Vale | N Kinseley |
| 26/12/2017 | Crawley Town | 0 | 2 | Colchester | A Coggins |
| 30/12/2017 | Chesterfield | 0 | 0 | Colchester | S Stockbridge |
| 01/01/2018 | Colchester | 0 | 0 | Cambridge | C Breakspear |
| 06/01/2018 | Colchester | 1 | 4 | Cheltenham | S Oldham |
| 13/01/2018 | Wycombe | 3 | 1 | Colchester | J Busby |
| 20/01/2018 | Colchester | 1 | 1 | Grimsby | C Hicks |
| 27/01/2018 | Port Vale | 2 | 2 | Colchester | T Kettle |
| 03/02/2018 | Colchester | 2 | 0 | Newport County | G Ward |
| 10/02/2018 | Carlisle | 1 | 1 | Colchester | R Joyce |
| 13/02/2018 | Colchester | 2 | 1 | Coventry | J Busby |
| 17/02/2018 | Crewe | 1 | 0 | Colchester | M Salisbury |
| 24/02/2018 | Colchester | 0 | 1 | Barnet | D Whitestone |
| 10/03/2018 | Mansfield | 1 | 1 | Colchester | R Joyce |
| 17/03/2018 | Colchester | 0 | 1 | Yeovil | T Nield |
| 20/03/2018 | Morecambe | 0 | 0 | Colchester | R Clark |
| 24/03/2018 | Stevenage | 0 | 1 | Colchester | G Salisbury |
| 30/03/2018 | Colchester | 2 | 1 | Luton | T Kettle |
| 02/04/2018 | Forest Green | 1 | 2 | Colchester | B Huxtable |
| 07/04/2018 | Colchester | 0 | 1 | Accrington | K Johnson |
| 14/04/2018 | Colchester | 1 | 3 | Notts County | B Malone |
| 21/04/2018 | Lincoln | 2 | 1 | Colchester | D Handley |
| 28/04/2018 | Colchester | 0 | 0 | Swindon | C Hicks |
| 05/05/2018 | Exeter | 1 | 0 | Colchester | C Breakspear |