Key: Win : Draw : Loss
| Date | Home | Away | Ref | ||
|---|---|---|---|---|---|
| 07/08/2010 | Tranmere | 1 | 2 | Oldham | G Salisbury |
| 14/08/2010 | Huddersfield | 0 | 0 | Tranmere | G Eltringham |
| 21/08/2010 | Tranmere | 0 | 3 | Bournemouth | C Boyeson |
| 28/08/2010 | Dag and Red | 2 | 2 | Tranmere | K Stroud |
| 04/09/2010 | Tranmere | 1 | 0 | Peterboro | S Rushton |
| 11/09/2010 | Yeovil | 3 | 1 | Tranmere | R East |
| 18/09/2010 | Tranmere | 1 | 1 | Charlton | P Gibbs |
| 25/09/2010 | Colchester | 3 | 1 | Tranmere | P Crossley |
| 28/09/2010 | Bristol Rvs | 0 | 1 | Tranmere | C Sarginson |
| 02/10/2010 | Tranmere | 1 | 1 | Brighton | N Miller |
| 09/10/2010 | Southampton | 2 | 0 | Tranmere | G Ward |
| 16/10/2010 | Tranmere | 0 | 3 | Brentford | D Webb |
| 23/10/2010 | Walsall | 1 | 4 | Tranmere | J Moss |
| 30/10/2010 | Tranmere | 4 | 2 | Milton Keynes Dons | C Webster |
| 02/11/2010 | Carlisle | 2 | 0 | Tranmere | E Ilderton |
| 13/11/2010 | Tranmere | 1 | 0 | Plymouth | R Madley |
| 20/11/2010 | Notts County | 0 | 1 | Tranmere | G Eltringham |
| 23/11/2010 | Tranmere | 0 | 1 | Hartlepool | G Sutton |
| 11/12/2010 | Tranmere | 1 | 2 | Leyton Orient | M Russell |
| 01/01/2011 | Rochdale | 3 | 2 | Tranmere | C Boyeson |
| 03/01/2011 | Tranmere | 2 | 1 | Carlisle | D Foster |
| 08/01/2011 | Tranmere | 3 | 3 | Walsall | M Naylor |
| 15/01/2011 | Milton Keynes Dons | 2 | 0 | Tranmere | K Wright |
| 22/01/2011 | Tranmere | 2 | 0 | Southampton | D Webb |
| 25/01/2011 | Swindon | 0 | 0 | Tranmere | J Linington |
| 01/02/2011 | Tranmere | 1 | 1 | Rochdale | D Drysdale |
| 12/02/2011 | Plymouth | 1 | 3 | Tranmere | G Horwood |
| 15/02/2011 | Tranmere | 3 | 0 | Sheffield Weds | N Swarbrick |
| 19/02/2011 | Peterboro | 2 | 1 | Tranmere | G Hegley |
| 22/02/2011 | Brentford | 2 | 1 | Tranmere | P Miller |
| 26/02/2011 | Tranmere | 0 | 1 | Yeovil | M Brown |
| 01/03/2011 | Exeter | 1 | 1 | Tranmere | S Hooper |
| 05/03/2011 | Charlton | 1 | 1 | Tranmere | D Coote |
| 08/03/2011 | Tranmere | 0 | 1 | Bristol Rvs | C Sarginson |
| 12/03/2011 | Brighton | 2 | 0 | Tranmere | S Rushton |
| 19/03/2011 | Tranmere | 1 | 0 | Colchester | A Woolmer |
| 28/03/2011 | Oldham | 0 | 0 | Tranmere | A Bates |
| 02/04/2011 | Tranmere | 0 | 2 | Huddersfield | D Deadman |
| 05/04/2011 | Sheffield Weds | 4 | 0 | Tranmere | D Whitestone |
| 09/04/2011 | Bournemouth | 1 | 2 | Tranmere | O Langford |
| 16/04/2011 | Tranmere | 2 | 0 | Dag and Red | D Coote |
| 19/04/2011 | Tranmere | 0 | 1 | Notts County | P Quinn |
| 22/04/2011 | Hartlepool | 1 | 1 | Tranmere | M Haywood |
| 25/04/2011 | Tranmere | 4 | 0 | Exeter | G Scott |
| 30/04/2011 | Leyton Orient | 0 | 3 | Tranmere | G Horwood |
| 07/05/2011 | Tranmere | 0 | 2 | Swindon | N Miller |