Key: Win : Draw : Loss
| Date | Home | Away | Ref | ||
|---|---|---|---|---|---|
| 07/08/2010 | Exeter | 2 | 2 | Colchester | P Gibbs |
| 14/08/2010 | Colchester | 1 | 1 | Sheffield Weds | D Phillips |
| 21/08/2010 | Rochdale | 1 | 2 | Colchester | N Miller |
| 27/08/2010 | Colchester | 1 | 1 | Carlisle | G Ward |
| 04/09/2010 | Walsall | 0 | 1 | Colchester | K Evans |
| 11/09/2010 | Colchester | 1 | 1 | Plymouth | D McDermid |
| 18/09/2010 | Southampton | 0 | 0 | Colchester | G Horwood |
| 25/09/2010 | Colchester | 3 | 1 | Tranmere | P Crossley |
| 28/09/2010 | Colchester | 2 | 2 | Dag and Red | K Hill |
| 02/10/2010 | Milton Keynes Dons | 1 | 1 | Colchester | M Russell |
| 09/10/2010 | Colchester | 0 | 3 | Huddersfield | P Miller |
| 16/10/2010 | Oldham | 0 | 0 | Colchester | P Quinn |
| 23/10/2010 | Colchester | 2 | 1 | Notts County | D Sheldrake |
| 30/10/2010 | Bournemouth | 1 | 2 | Colchester | S Hooper |
| 02/11/2010 | Colchester | 3 | 2 | Leyton Orient | G Hegley |
| 13/11/2010 | Swindon | 2 | 1 | Colchester | R Lewis |
| 20/11/2010 | Colchester | 3 | 2 | Hartlepool | O Langford |
| 23/11/2010 | Colchester | 0 | 2 | Brentford | D Foster |
| 11/12/2010 | Colchester | 0 | 0 | Yeovil | S Rushton |
| 01/01/2011 | Colchester | 3 | 3 | Charlton | P Miller |
| 03/01/2011 | Leyton Orient | 4 | 2 | Colchester | D McDermid |
| 14/01/2011 | Colchester | 2 | 1 | Bournemouth | D Deadman |
| 22/01/2011 | Huddersfield | 0 | 0 | Colchester | G Ward |
| 25/01/2011 | Brighton | 2 | 0 | Colchester | G Scott |
| 28/01/2011 | Colchester | 2 | 1 | Peterboro | P Crossley |
| 01/02/2011 | Charlton | 1 | 0 | Colchester | D Sheldrake |
| 05/02/2011 | Hartlepool | 1 | 0 | Colchester | T Kettle |
| 12/02/2011 | Colchester | 2 | 1 | Swindon | G Salisbury |
| 15/02/2011 | Notts County | 2 | 0 | Colchester | C Webster |
| 19/02/2011 | Colchester | 2 | 0 | Walsall | M Russell |
| 22/02/2011 | Peterboro | 1 | 1 | Colchester | D Drysdale |
| 26/02/2011 | Plymouth | 2 | 1 | Colchester | O Langford |
| 01/03/2011 | Bristol Rvs | 0 | 1 | Colchester | M Naylor |
| 05/03/2011 | Colchester | 0 | 2 | Southampton | P Gibbs |
| 08/03/2011 | Dag and Red | 1 | 0 | Colchester | G Ward |
| 12/03/2011 | Colchester | 1 | 3 | Milton Keynes Dons | C Sarginson |
| 19/03/2011 | Tranmere | 1 | 0 | Colchester | A Woolmer |
| 22/03/2011 | Colchester | 1 | 0 | Oldham | J Linnington |
| 26/03/2011 | Colchester | 5 | 1 | Exeter | K Evans |
| 02/04/2011 | Sheffield Weds | 2 | 1 | Colchester | S Mathieson |
| 09/04/2011 | Colchester | 1 | 0 | Rochdale | R East |
| 16/04/2011 | Carlisle | 4 | 1 | Colchester | P Quinn |
| 22/04/2011 | Brentford | 1 | 1 | Colchester | G Horwood |
| 25/04/2011 | Colchester | 1 | 1 | Brighton | K Stroud |
| 30/04/2011 | Yeovil | 4 | 2 | Colchester | O Langford |
| 07/05/2011 | Colchester | 2 | 1 | Bristol Rvs | D Webb |