Key: Win : Draw : Loss
| Date | Home | Away | Ref | ||
|---|---|---|---|---|---|
| 08/08/2009 | Yeovil | 2 | 0 | Tranmere | A Hall |
| 15/08/2009 | Colchester | 2 | 1 | Yeovil | R Booth |
| 18/08/2009 | Exeter | 1 | 1 | Yeovil | C Sarginson |
| 22/08/2009 | Yeovil | 3 | 3 | Leyton Orient | S Bratt |
| 29/08/2009 | Huddersfield | 2 | 1 | Yeovil | S Hooper |
| 05/09/2009 | Yeovil | 0 | 1 | Swindon | J Singh |
| 12/09/2009 | Yeovil | 2 | 2 | Stockport | G Scott |
| 19/09/2009 | Southampton | 2 | 0 | Yeovil | A Bates |
| 26/09/2009 | Yeovil | 2 | 0 | Brentford | R East |
| 29/09/2009 | Millwall | 0 | 0 | Yeovil | S Cook |
| 03/10/2009 | Oldham | 0 | 0 | Yeovil | N Miller |
| 10/10/2009 | Yeovil | 2 | 2 | Brighton | O Langford |
| 17/10/2009 | Yeovil | 3 | 1 | Carlisle | F Graham |
| 24/10/2009 | Bristol Rvs | 1 | 2 | Yeovil | N Swarbrick |
| 31/10/2009 | Leeds | 4 | 0 | Yeovil | A Haines |
| 14/11/2009 | Yeovil | 1 | 0 | Southend | D Phillips |
| 21/11/2009 | Yeovil | 1 | 1 | Charlton | S Hooper |
| 24/11/2009 | Gillingham | 1 | 0 | Yeovil | P Miller |
| 01/12/2009 | Yeovil | 1 | 3 | Walsall | J Linington |
| 05/12/2009 | Milton Keynes Dons | 2 | 2 | Yeovil | P Tierney |
| 12/12/2009 | Yeovil | 3 | 3 | Norwich | C Pawson |
| 19/12/2009 | Hartlepool | 1 | 1 | Yeovil | R Booth |
| 26/12/2009 | Yeovil | 4 | 0 | Wycombe | R East |
| 28/12/2009 | Swindon | 3 | 1 | Yeovil | K Stroud |
| 19/01/2010 | Leyton Orient | 2 | 0 | Yeovil | P Gibbs |
| 23/01/2010 | Yeovil | 2 | 1 | Exeter | S Tanner |
| 26/01/2010 | Tranmere | 2 | 1 | Yeovil | G Salisbury |
| 30/01/2010 | Yeovil | 0 | 1 | Huddersfield | G Ward |
| 06/02/2010 | Wycombe | 1 | 4 | Yeovil | S Bratt |
| 13/02/2010 | Yeovil | 0 | 0 | Gillingham | P Crossley |
| 16/02/2010 | Yeovil | 0 | 1 | Colchester | D Sheldrake |
| 20/02/2010 | Charlton | 2 | 0 | Yeovil | F Graham |
| 23/02/2010 | Walsall | 0 | 1 | Yeovil | G Sutton |
| 27/02/2010 | Yeovil | 1 | 0 | Milton Keynes Dons | G Salisbury |
| 06/03/2010 | Norwich | 3 | 0 | Yeovil | J Linington |
| 13/03/2010 | Yeovil | 4 | 0 | Hartlepool | S Rushton |
| 16/03/2010 | Carlisle | 1 | 0 | Yeovil | A Bates |
| 20/03/2010 | Yeovil | 0 | 3 | Bristol Rvs | M Haywood |
| 03/04/2010 | Southend | 0 | 0 | Yeovil | R Booth |
| 05/04/2010 | Yeovil | 1 | 2 | Leeds | K Wright |
| 10/04/2010 | Stockport | 1 | 3 | Yeovil | G Eltrigham |
| 13/04/2010 | Yeovil | 1 | 1 | Millwall | M Russell |
| 17/04/2010 | Yeovil | 0 | 1 | Southampton | O Langford |
| 24/04/2010 | Brentford | 1 | 1 | Yeovil | G Horwood |
| 01/05/2010 | Yeovil | 3 | 0 | Oldham | J Singh |
| 08/05/2010 | Brighton | 1 | 0 | Yeovil | S Tanner |