Key: Win : Draw : Loss
| Date | Home | Away | Ref | ||
|---|---|---|---|---|---|
| 08/08/2009 | Rotherham | 1 | 0 | Accrington | D McDermid |
| 15/08/2009 | Bournemouth | 1 | 0 | Rotherham | S Cook |
| 18/08/2009 | Grimsby | 1 | 2 | Rotherham | G Salisbury |
| 23/08/2009 | Rotherham | 2 | 1 | Rochdale | G Sutton |
| 29/08/2009 | Macclesfield | 1 | 3 | Rotherham | C Sarginson |
| 05/09/2009 | Rotherham | 3 | 1 | Chesterfield | N Miller |
| 12/09/2009 | Rotherham | 0 | 0 | Morecambe | D Deadman |
| 19/09/2009 | Cheltenham | 1 | 1 | Rotherham | P Tierney |
| 26/09/2009 | Rotherham | 3 | 0 | Barnet | P Quinn |
| 29/09/2009 | Northampton | 3 | 1 | Rotherham | O Langford |
| 03/10/2009 | Crewe | 2 | 3 | Rotherham | T Kettle |
| 10/10/2009 | Rotherham | 1 | 1 | Hereford | N Swarbrick |
| 17/10/2009 | Rotherham | 0 | 0 | Notts County | C Boyeson |
| 24/10/2009 | Bury | 2 | 1 | Rotherham | D Webb |
| 31/10/2009 | Rotherham | 0 | 0 | Aldershot | R Booth |
| 14/11/2009 | Port Vale | 1 | 2 | Rotherham | P Quinn |
| 21/11/2009 | Torquay | 0 | 2 | Rotherham | A Hall |
| 24/11/2009 | Rotherham | 2 | 0 | Lincoln | M Russell |
| 01/12/2009 | Shrewsbury | 2 | 0 | Rotherham | S Rushton |
| 05/12/2009 | Rotherham | 2 | 2 | Burton | S Hooper |
| 12/12/2009 | Bradford | 2 | 4 | Rotherham | L Probert |
| 19/01/2010 | Rotherham | 1 | 2 | Darlington | A Taylor |
| 23/01/2010 | Rotherham | 2 | 1 | Grimsby | E Ilderton |
| 26/01/2010 | Chesterfield | 0 | 1 | Rotherham | S Mathieson |
| 02/02/2010 | Rotherham | 1 | 3 | Bournemouth | N Swarbrick |
| 06/02/2010 | Darlington | 2 | 0 | Rotherham | D Whitestone |
| 09/02/2010 | Rotherham | 2 | 0 | Dag and Red | K Evans |
| 13/02/2010 | Lincoln | 1 | 2 | Rotherham | J Singh |
| 19/02/2010 | Rotherham | 1 | 1 | Torquay | N Miller |
| 22/02/2010 | Rotherham | 1 | 1 | Shrewsbury | D Foster |
| 27/02/2010 | Burton | 0 | 1 | Rotherham | K Stroud |
| 02/03/2010 | Rochdale | 4 | 0 | Rotherham | S Tanner |
| 06/03/2010 | Rotherham | 1 | 2 | Bradford | A Haines |
| 13/03/2010 | Dag and Red | 0 | 1 | Rotherham | J Linington |
| 16/03/2010 | Accrington | 2 | 1 | Rotherham | G Sutton |
| 20/03/2010 | Rotherham | 1 | 0 | Bury | M Russell |
| 23/03/2010 | Rotherham | 3 | 1 | Macclesfield | G Salisbury |
| 27/03/2010 | Notts County | 1 | 0 | Rotherham | G Hegley |
| 03/04/2010 | Rotherham | 1 | 2 | Port Vale | M Halsey |
| 05/04/2010 | Aldershot | 3 | 0 | Rotherham | K Friend |
| 10/04/2010 | Morecambe | 2 | 0 | Rotherham | R Shoebridge |
| 13/04/2010 | Rotherham | 1 | 0 | Northampton | K Evans |
| 17/04/2010 | Rotherham | 0 | 0 | Cheltenham | G Horwood |
| 24/04/2010 | Barnet | 0 | 1 | Rotherham | S Rushton |
| 01/05/2010 | Rotherham | 0 | 0 | Crewe | R Booth |
| 08/05/2010 | Hereford | 3 | 0 | Rotherham | E Ilderton |