Key: Win : Draw : Loss
| Date | Home | Away | Ref | ||
|---|---|---|---|---|---|
| 08/08/2009 | Derby | 2 | 1 | Peterboro | P Taylor |
| 15/08/2009 | Peterboro | 1 | 1 | Sheffield Weds | A D'Urso |
| 18/08/2009 | Peterboro | 2 | 3 | West Brom | G Horwood |
| 22/08/2009 | Preston | 2 | 0 | Peterboro | D Webb |
| 31/08/2009 | Peterboro | 1 | 1 | Crystal Palace | K Friend |
| 12/09/2009 | QPR | 1 | 1 | Peterboro | O Langford |
| 15/09/2009 | Leicester | 1 | 1 | Peterboro | R Booth |
| 19/09/2009 | Peterboro | 3 | 2 | Reading | C Sarginson |
| 26/09/2009 | Blackpool | 2 | 0 | Peterboro | A Bates |
| 29/09/2009 | Peterboro | 1 | 2 | Plymouth | A Hall |
| 03/10/2009 | Peterboro | 1 | 2 | Nott'm Forest | J Moss |
| 17/10/2009 | Bristol City | 1 | 1 | Peterboro | K Hill |
| 20/10/2009 | Doncaster | 3 | 1 | Peterboro | C Webster |
| 24/10/2009 | Peterboro | 3 | 0 | Scunthorpe | M Jones |
| 31/10/2009 | Peterboro | 1 | 2 | Barnsley | C Boyeson |
| 07/11/2009 | Newcastle | 3 | 1 | Peterboro | A D'Urso |
| 21/11/2009 | Sheffield United | 1 | 0 | Peterboro | G Hegley |
| 28/11/2009 | Peterboro | 2 | 2 | Middlesbrough | D Phillips |
| 05/12/2009 | Peterboro | 2 | 2 | Swansea | R Booth |
| 08/12/2009 | Ipswich | 0 | 0 | Peterboro | A Hall |
| 12/12/2009 | Coventry | 3 | 2 | Peterboro | R East |
| 19/12/2009 | Peterboro | 2 | 1 | Watford | K Evans |
| 26/12/2009 | West Brom | 2 | 0 | Peterboro | M Jones |
| 28/12/2009 | Peterboro | 4 | 4 | Cardiff | D Whitestone |
| 16/01/2010 | Peterboro | 0 | 3 | Derby | N Miller |
| 23/01/2010 | Sheffield Weds | 2 | 1 | Peterboro | D Foster |
| 26/01/2010 | Peterboro | 0 | 1 | Preston | D Sheldrake |
| 30/01/2010 | Crystal Palace | 2 | 0 | Peterboro | G Salisbury |
| 06/02/2010 | Peterboro | 1 | 0 | QPR | D McDermid |
| 09/02/2010 | Cardiff | 2 | 0 | Peterboro | A Penn |
| 13/02/2010 | Middlesbrough | 1 | 0 | Peterboro | J Moss |
| 16/02/2010 | Peterboro | 3 | 1 | Ipswich | J Linington |
| 27/02/2010 | Swansea | 1 | 0 | Peterboro | G Ward |
| 06/03/2010 | Peterboro | 0 | 1 | Coventry | R Booth |
| 09/03/2010 | Peterboro | 1 | 0 | Sheffield United | J Singh |
| 13/03/2010 | Watford | 0 | 1 | Peterboro | A Taylor |
| 16/03/2010 | Peterboro | 1 | 2 | Doncaster | F Graham |
| 20/03/2010 | Nott'm Forest | 1 | 0 | Peterboro | K Hill |
| 23/03/2010 | Scunthorpe | 4 | 0 | Peterboro | E Ilderton |
| 27/03/2010 | Peterboro | 0 | 1 | Bristol City | D Webb |
| 03/04/2010 | Peterboro | 2 | 3 | Newcastle | M Haywood |
| 05/04/2010 | Barnsley | 2 | 2 | Peterboro | A Bates |
| 10/04/2010 | Peterboro | 1 | 2 | Leicester | G Scott |
| 17/04/2010 | Reading | 6 | 0 | Peterboro | C Boyeson |
| 24/04/2010 | Peterboro | 0 | 1 | Blackpool | C Webster |
| 02/05/2010 | Plymouth | 1 | 2 | Peterboro | S Tanner |