Key: Win : Draw : Loss
| Date | Home | Away | Ref | ||
|---|---|---|---|---|---|
| 08/08/2009 | Northampton | 0 | 0 | Macclesfield | G Ward |
| 15/08/2009 | Macclesfield | 0 | 4 | Notts County | P Tierney |
| 18/08/2009 | Macclesfield | 2 | 0 | Port Vale | J Singh |
| 22/08/2009 | Morecambe | 2 | 2 | Macclesfield | P Quinn |
| 29/08/2009 | Macclesfield | 1 | 3 | Rotherham | C Sarginson |
| 05/09/2009 | Crewe | 2 | 1 | Macclesfield | S Bratt |
| 12/09/2009 | Macclesfield | 1 | 1 | Barnet | G Sutton |
| 19/09/2009 | Chesterfield | 4 | 1 | Macclesfield | G Eltringham |
| 26/09/2009 | Macclesfield | 2 | 1 | Torquay | N Swarbrick |
| 29/09/2009 | Burton | 1 | 1 | Macclesfield | J Moss |
| 03/10/2009 | Darlington | 0 | 1 | Macclesfield | C Pawson |
| 10/10/2009 | Macclesfield | 0 | 1 | Lincoln | E Evans |
| 16/10/2009 | Cheltenham | 1 | 2 | Macclesfield | D Phillips |
| 24/10/2009 | Macclesfield | 2 | 2 | Dag and Red | O Langford |
| 31/10/2009 | Macclesfield | 2 | 2 | Bradford | R Shoebridge |
| 14/11/2009 | Aldershot | 0 | 0 | Macclesfield | S Tanner |
| 21/11/2009 | Macclesfield | 1 | 2 | Bournemouth | T Kettle |
| 28/11/2009 | Macclesfield | 0 | 0 | Grimsby | A Woolmer |
| 05/12/2009 | Rochdale | 3 | 0 | Macclesfield | S Cook |
| 12/12/2009 | Macclesfield | 3 | 1 | Hereford | S Bratt |
| 26/12/2009 | Shrewsbury | 2 | 2 | Macclesfield | P Tierney |
| 28/12/2009 | Macclesfield | 4 | 1 | Crewe | J Waugh |
| 20/01/2010 | Macclesfield | 0 | 2 | Northampton | D McDermid |
| 23/01/2010 | Port Vale | 0 | 0 | Macclesfield | P Miller |
| 26/01/2010 | Macclesfield | 2 | 2 | Morecambe | D Whitestone |
| 06/02/2010 | Macclesfield | 0 | 1 | Shrewsbury | J Linington |
| 09/02/2010 | Bury | 2 | 1 | Macclesfield | C Boyeson |
| 13/02/2010 | Macclesfield | 0 | 0 | Accrington | P Quinn |
| 20/02/2010 | Bournemouth | 1 | 1 | Macclesfield | G Horwood |
| 23/02/2010 | Grimsby | 1 | 1 | Macclesfield | O Langford |
| 27/02/2010 | Macclesfield | 0 | 1 | Rochdale | M Haywood |
| 02/03/2010 | Notts County | 1 | 0 | Macclesfield | S Rushton |
| 06/03/2010 | Hereford | 0 | 2 | Macclesfield | G Ward |
| 09/03/2010 | Accrington | 1 | 1 | Macclesfield | A Haines |
| 13/03/2010 | Macclesfield | 2 | 0 | Bury | K Wright |
| 20/03/2010 | Dag and Red | 3 | 1 | Macclesfield | A Bates |
| 23/03/2010 | Rotherham | 3 | 1 | Macclesfield | G Salisbury |
| 27/03/2010 | Macclesfield | 1 | 0 | Cheltenham | E Ilderton |
| 03/04/2010 | Macclesfield | 1 | 1 | Aldershot | D Webb |
| 05/04/2010 | Bradford | 1 | 2 | Macclesfield | R Booth |
| 10/04/2010 | Barnet | 1 | 2 | Macclesfield | S Tanner |
| 13/04/2010 | Macclesfield | 1 | 1 | Burton | A Penn |
| 17/04/2010 | Macclesfield | 2 | 0 | Chesterfield | D Sheldrake |
| 24/04/2010 | Torquay | 1 | 0 | Macclesfield | G Scott |
| 01/05/2010 | Macclesfield | 0 | 2 | Darlington | P Miller |
| 08/05/2010 | Lincoln | 0 | 0 | Macclesfield | A Haines |