Key: Win : Draw : Loss
| Date | Home | Away | Ref | ||
|---|---|---|---|---|---|
| 08/08/2009 | Lincoln | 1 | 0 | Barnet | T Kettle |
| 15/08/2009 | Accrington | 1 | 0 | Lincoln | O Langford |
| 18/08/2009 | Bradford | 0 | 2 | Lincoln | D Webb |
| 22/08/2009 | Lincoln | 0 | 2 | Burton | G Eltringham |
| 29/08/2009 | Dag and Red | 3 | 0 | Lincoln | K Hill |
| 05/09/2009 | Lincoln | 3 | 0 | Darlington | D Whitestone |
| 12/09/2009 | Bournemouth | 3 | 1 | Lincoln | J Linington |
| 19/09/2009 | Lincoln | 0 | 2 | Shrewsbury | P Quinn |
| 26/09/2009 | Bury | 2 | 0 | Lincoln | J Moss |
| 29/09/2009 | Lincoln | 0 | 3 | Notts County | C Pawson |
| 03/10/2009 | Lincoln | 1 | 0 | Aldershot | D Foster |
| 10/10/2009 | Macclesfield | 0 | 1 | Lincoln | E Evans |
| 17/10/2009 | Northampton | 1 | 0 | Lincoln | S Cook |
| 24/10/2009 | Lincoln | 0 | 0 | Torquay | D McDermid |
| 31/10/2009 | Morecambe | 3 | 1 | Lincoln | P Tierney |
| 14/11/2009 | Lincoln | 1 | 1 | Cheltenham | G Salisbury |
| 21/11/2009 | Lincoln | 0 | 0 | Grimsby | S Tanner |
| 24/11/2009 | Rotherham | 2 | 0 | Lincoln | M Russell |
| 01/12/2009 | Lincoln | 1 | 2 | Port Vale | R Booth |
| 05/12/2009 | Crewe | 0 | 0 | Lincoln | M Haywood |
| 12/12/2009 | Lincoln | 1 | 3 | Rochdale | A Woolmer |
| 26/12/2009 | Lincoln | 2 | 1 | Chesterfield | S Rushton |
| 16/01/2010 | Barnet | 1 | 2 | Lincoln | J Waugh |
| 23/01/2010 | Lincoln | 2 | 1 | Bradford | S Bratt |
| 27/01/2010 | Burton | 1 | 0 | Lincoln | G Horwood |
| 02/02/2010 | Hereford | 2 | 0 | Lincoln | R East |
| 06/02/2010 | Chesterfield | 2 | 1 | Lincoln | D Webb |
| 09/02/2010 | Darlington | 1 | 1 | Lincoln | A Haines |
| 13/02/2010 | Lincoln | 1 | 2 | Rotherham | J Singh |
| 16/02/2010 | Lincoln | 2 | 1 | Accrington | R Shoebridge |
| 20/02/2010 | Grimsby | 2 | 2 | Lincoln | M Haywood |
| 23/02/2010 | Port Vale | 4 | 0 | Lincoln | P Gibbs |
| 27/02/2010 | Lincoln | 1 | 1 | Crewe | E Ilderton |
| 06/03/2010 | Rochdale | 1 | 1 | Lincoln | D Foster |
| 12/03/2010 | Lincoln | 3 | 1 | Hereford | D Deadman |
| 16/03/2010 | Lincoln | 1 | 1 | Dag and Red | S Mathieson |
| 20/03/2010 | Torquay | 2 | 3 | Lincoln | C Pawson |
| 27/03/2010 | Lincoln | 1 | 1 | Northampton | J Moss |
| 03/04/2010 | Cheltenham | 1 | 0 | Lincoln | S Hooper |
| 05/04/2010 | Lincoln | 1 | 3 | Morecambe | S Cook |
| 10/04/2010 | Lincoln | 2 | 1 | Bournemouth | P Tierney |
| 13/04/2010 | Notts County | 3 | 1 | Lincoln | P Quinn |
| 17/04/2010 | Shrewsbury | 1 | 0 | Lincoln | J Waugh |
| 24/04/2010 | Lincoln | 1 | 0 | Bury | J Singh |
| 01/05/2010 | Aldershot | 3 | 1 | Lincoln | D Phillips |
| 08/05/2010 | Lincoln | 0 | 0 | Macclesfield | A Haines |