Key: Win : Draw : Loss
| Date | Home | Away | Ref | ||
|---|---|---|---|---|---|
| 08/08/2009 | Milton Keynes Dons | 0 | 0 | Hartlepool | K Hill |
| 15/08/2009 | Hartlepool | 0 | 2 | Charlton | C Boyeson |
| 18/08/2009 | Hartlepool | 1 | 2 | Bristol Rvs | K Evans |
| 22/08/2009 | Gillingham | 0 | 1 | Hartlepool | D McDermid |
| 29/08/2009 | Hartlepool | 0 | 2 | Norwich | S Mathieson |
| 04/09/2009 | Oldham | 0 | 3 | Hartlepool | R Shoebridge |
| 12/09/2009 | Hartlepool | 1 | 1 | Wycombe | A Taylor |
| 19/09/2009 | Colchester | 2 | 0 | Hartlepool | A Woolmer |
| 26/09/2009 | Hartlepool | 3 | 0 | Walsall | D Foster |
| 29/09/2009 | Stockport | 2 | 2 | Hartlepool | C Sarginson |
| 03/10/2009 | Exeter | 3 | 1 | Hartlepool | J Linington |
| 09/10/2009 | Hartlepool | 0 | 0 | Brentford | G Salisbury |
| 17/10/2009 | Swindon | 0 | 2 | Hartlepool | T Kettle |
| 24/10/2009 | Hartlepool | 1 | 0 | Tranmere | G Sutton |
| 31/10/2009 | Brighton | 3 | 3 | Hartlepool | P Miller |
| 14/11/2009 | Hartlepool | 1 | 0 | Leyton Orient | C Pawson |
| 21/11/2009 | Huddersfield | 2 | 1 | Hartlepool | P Tierney |
| 24/11/2009 | Hartlepool | 1 | 3 | Southampton | N Swarbrick |
| 01/12/2009 | Carlisle | 3 | 2 | Hartlepool | A Taylor |
| 05/12/2009 | Hartlepool | 3 | 0 | Millwall | J Waugh |
| 12/12/2009 | Southend | 3 | 2 | Hartlepool | P Gibbs |
| 19/12/2009 | Hartlepool | 1 | 1 | Yeovil | R Booth |
| 26/12/2009 | Leeds | 3 | 1 | Hartlepool | E Ilderton |
| 02/01/2010 | Hartlepool | 2 | 1 | Oldham | C Boyeson |
| 16/01/2010 | Hartlepool | 0 | 5 | Milton Keynes Dons | C Sarginson |
| 19/01/2010 | Charlton | 2 | 1 | Hartlepool | S Tanner |
| 23/01/2010 | Bristol Rvs | 2 | 0 | Hartlepool | G Ward |
| 26/01/2010 | Hartlepool | 1 | 1 | Gillingham | K Hill |
| 30/01/2010 | Norwich | 2 | 1 | Hartlepool | P Taylor |
| 06/02/2010 | Hartlepool | 2 | 2 | Leeds | S Mathieson |
| 20/02/2010 | Hartlepool | 0 | 2 | Huddersfield | A Taylor |
| 23/02/2010 | Hartlepool | 4 | 1 | Carlisle | C Webster |
| 27/02/2010 | Millwall | 1 | 0 | Hartlepool | M Russell |
| 06/03/2010 | Hartlepool | 3 | 0 | Southend | J Waugh |
| 13/03/2010 | Yeovil | 4 | 0 | Hartlepool | S Rushton |
| 19/03/2010 | Tranmere | 0 | 0 | Hartlepool | K Evans |
| 23/03/2010 | Southampton | 3 | 2 | Hartlepool | A D'Urso |
| 27/03/2010 | Hartlepool | 0 | 1 | Swindon | M Oliver |
| 03/04/2010 | Leyton Orient | 1 | 3 | Hartlepool | G Sutton |
| 05/04/2010 | Hartlepool | 2 | 0 | Brighton | M Haywood |
| 10/04/2010 | Wycombe | 2 | 0 | Hartlepool | D Sheldrake |
| 13/04/2010 | Hartlepool | 3 | 0 | Stockport | A Hall |
| 17/04/2010 | Hartlepool | 3 | 1 | Colchester | J Moss |
| 24/04/2010 | Walsall | 3 | 1 | Hartlepool | F Graham |
| 01/05/2010 | Hartlepool | 1 | 1 | Exeter | G Salisbury |
| 08/05/2010 | Brentford | 0 | 0 | Hartlepool | D McDermid |