Key: Win : Draw : Loss
| Date | Home | Away | Ref | ||
|---|---|---|---|---|---|
| 08/08/2009 | Cheltenham | 2 | 1 | Grimsby | D Sheldrake |
| 15/08/2009 | Grimsby | 0 | 4 | Crewe | M Haywood |
| 18/08/2009 | Grimsby | 1 | 2 | Rotherham | G Salisbury |
| 22/08/2009 | Bury | 0 | 1 | Grimsby | R Booth |
| 29/08/2009 | Grimsby | 1 | 2 | Aldershot | P Quinn |
| 05/09/2009 | Port Vale | 4 | 0 | Grimsby | G Eltringham |
| 12/09/2009 | Grimsby | 1 | 0 | Hereford | J Waugh |
| 19/09/2009 | Torquay | 0 | 2 | Grimsby | O Langford |
| 26/09/2009 | Grimsby | 1 | 1 | Darlington | S Mathieson |
| 30/09/2009 | Chesterfield | 3 | 2 | Grimsby | D McDermid |
| 03/10/2009 | Barnet | 3 | 0 | Grimsby | D Sheldrake |
| 10/10/2009 | Grimsby | 1 | 2 | Burton | S Rushton |
| 17/10/2009 | Grimsby | 0 | 2 | Rochdale | J Moss |
| 24/10/2009 | Bournemouth | 3 | 1 | Grimsby | P Gibbs |
| 30/10/2009 | Grimsby | 2 | 2 | Accrington | D Webb |
| 14/11/2009 | Northampton | 0 | 0 | Grimsby | K Stroud |
| 21/11/2009 | Lincoln | 0 | 0 | Grimsby | S Tanner |
| 24/11/2009 | Grimsby | 0 | 3 | Bradford | D Deadman |
| 28/11/2009 | Macclesfield | 0 | 0 | Grimsby | A Woolmer |
| 05/12/2009 | Grimsby | 1 | 1 | Dag and Red | S Bratt |
| 12/12/2009 | Shrewsbury | 0 | 0 | Grimsby | A Hall |
| 18/12/2009 | Grimsby | 1 | 1 | Morecambe | C Pawson |
| 28/12/2009 | Grimsby | 1 | 2 | Port Vale | G Sutton |
| 02/01/2010 | Grimsby | 1 | 1 | Bury | G Eltringham |
| 16/01/2010 | Grimsby | 0 | 0 | Cheltenham | P Tierney |
| 23/01/2010 | Rotherham | 2 | 1 | Grimsby | E Ilderton |
| 30/01/2010 | Aldershot | 1 | 1 | Grimsby | K Evans |
| 06/02/2010 | Grimsby | 0 | 1 | Notts County | M Russell |
| 13/02/2010 | Bradford | 0 | 0 | Grimsby | S Tanner |
| 17/02/2010 | Notts County | 1 | 1 | Grimsby | K Wright |
| 20/02/2010 | Grimsby | 2 | 2 | Lincoln | M Haywood |
| 23/02/2010 | Grimsby | 1 | 1 | Macclesfield | O Langford |
| 27/02/2010 | Dag and Red | 2 | 0 | Grimsby | A Penn |
| 06/03/2010 | Grimsby | 3 | 0 | Shrewsbury | J Moss |
| 09/03/2010 | Crewe | 4 | 2 | Grimsby | C Webster |
| 13/03/2010 | Morecambe | 1 | 1 | Grimsby | D Webb |
| 20/03/2010 | Grimsby | 3 | 2 | Bournemouth | R Shoebridge |
| 27/03/2010 | Rochdale | 4 | 1 | Grimsby | F Graham |
| 02/04/2010 | Grimsby | 1 | 2 | Northampton | A Taylor |
| 05/04/2010 | Accrington | 2 | 3 | Grimsby | S Rushton |
| 10/04/2010 | Hereford | 0 | 1 | Grimsby | P Miller |
| 13/04/2010 | Grimsby | 2 | 2 | Chesterfield | K Wright |
| 17/04/2010 | Grimsby | 0 | 3 | Torquay | G Salisbury |
| 24/04/2010 | Darlington | 0 | 2 | Grimsby | M Haywood |
| 01/05/2010 | Grimsby | 2 | 0 | Barnet | N Miller |
| 08/05/2010 | Burton | 3 | 0 | Grimsby | A Bates |