Key: Win : Draw : Loss
| Date | Home | Away | Ref | ||
|---|---|---|---|---|---|
| 08/08/2009 | Aldershot | 3 | 1 | Darlington | G Sutton |
| 15/08/2009 | Darlington | 0 | 1 | Bury | C Sarginson |
| 18/08/2009 | Darlington | 0 | 1 | Crewe | J Waugh |
| 22/08/2009 | Port Vale | 1 | 0 | Darlington | P Tierney |
| 29/08/2009 | Darlington | 1 | 1 | Cheltenham | K Wright |
| 05/09/2009 | Lincoln | 3 | 0 | Darlington | D Whitestone |
| 11/09/2009 | Accrington | 2 | 1 | Darlington | A Penn |
| 19/09/2009 | Darlington | 0 | 2 | Bournemouth | C Boyeson |
| 26/09/2009 | Grimsby | 1 | 1 | Darlington | S Mathieson |
| 29/09/2009 | Darlington | 0 | 2 | Rochdale | S Rushton |
| 03/10/2009 | Darlington | 0 | 1 | Macclesfield | C Pawson |
| 10/10/2009 | Dag and Red | 2 | 0 | Darlington | D Deadman |
| 17/10/2009 | Darlington | 2 | 1 | Shrewsbury | A Haines |
| 24/10/2009 | Barnet | 3 | 0 | Darlington | S Bratt |
| 31/10/2009 | Hereford | 2 | 1 | Darlington | S Hooper |
| 14/11/2009 | Darlington | 1 | 0 | Burton | D Foster |
| 21/11/2009 | Chesterfield | 5 | 2 | Darlington | J Moss |
| 24/11/2009 | Darlington | 0 | 4 | Morecambe | K Evans |
| 01/12/2009 | Notts County | 4 | 0 | Darlington | G Salisbury |
| 05/12/2009 | Darlington | 0 | 1 | Bradford | N Swarbrick |
| 12/12/2009 | Torquay | 5 | 0 | Darlington | K Wright |
| 19/01/2010 | Rotherham | 1 | 2 | Darlington | A Taylor |
| 23/01/2010 | Crewe | 3 | 0 | Darlington | A Woolmer |
| 26/01/2010 | Darlington | 1 | 2 | Northampton | C Boyeson |
| 06/02/2010 | Darlington | 2 | 0 | Rotherham | D Whitestone |
| 09/02/2010 | Darlington | 1 | 1 | Lincoln | A Haines |
| 13/02/2010 | Morecambe | 2 | 0 | Darlington | G Eltringham |
| 20/02/2010 | Darlington | 2 | 3 | Chesterfield | G Sutton |
| 27/02/2010 | Bradford | 1 | 0 | Darlington | K Evans |
| 02/03/2010 | Darlington | 1 | 3 | Port Vale | M Haywood |
| 06/03/2010 | Darlington | 1 | 3 | Torquay | S Mathieson |
| 09/03/2010 | Bury | 1 | 1 | Darlington | A Hall |
| 13/03/2010 | Northampton | 2 | 0 | Darlington | A Penn |
| 20/03/2010 | Darlington | 1 | 2 | Barnet | C Sarginson |
| 23/03/2010 | Darlington | 1 | 2 | Aldershot | C Pawson |
| 27/03/2010 | Shrewsbury | 0 | 2 | Darlington | D Phillips |
| 03/04/2010 | Burton | 1 | 2 | Darlington | J Waugh |
| 05/04/2010 | Darlington | 0 | 1 | Hereford | P Tierney |
| 10/04/2010 | Darlington | 0 | 0 | Accrington | A Woolmer |
| 13/04/2010 | Rochdale | 0 | 1 | Darlington | O Langford |
| 17/04/2010 | Bournemouth | 2 | 0 | Darlington | P Taylor |
| 20/04/2010 | Cheltenham | 3 | 3 | Darlington | R East |
| 24/04/2010 | Darlington | 0 | 2 | Grimsby | M Haywood |
| 27/04/2010 | Darlington | 0 | 5 | Notts County | J Moss |
| 01/05/2010 | Macclesfield | 0 | 2 | Darlington | P Miller |
| 08/05/2010 | Darlington | 0 | 2 | Dag and Red | D Webb |