Key: Win : Draw : Loss
| Date | Home | Away | Ref | ||
|---|---|---|---|---|---|
| 08/08/2009 | Charlton | 3 | 2 | Wycombe | D Deadman |
| 15/08/2009 | Hartlepool | 0 | 2 | Charlton | C Boyeson |
| 18/08/2009 | Leyton Orient | 1 | 2 | Charlton | P Crossley |
| 22/08/2009 | Charlton | 2 | 0 | Walsall | S Hooper |
| 29/08/2009 | Tranmere | 0 | 4 | Charlton | P Tierney |
| 05/09/2009 | Charlton | 2 | 0 | Brentford | J Linnington |
| 12/09/2009 | Charlton | 1 | 1 | Southampton | K Wright |
| 19/09/2009 | Norwich | 2 | 2 | Charlton | S Tanner |
| 26/09/2009 | Charlton | 2 | 1 | Exeter | G Hegley |
| 29/09/2009 | Colchester | 3 | 0 | Charlton | P Gibbs |
| 03/10/2009 | Leeds | 0 | 0 | Charlton | S Mathieson |
| 10/10/2009 | Charlton | 0 | 0 | Oldham | G Horwood |
| 17/10/2009 | Charlton | 2 | 1 | Huddersfield | R East |
| 24/10/2009 | Gillingham | 1 | 1 | Charlton | J Moss |
| 31/10/2009 | Carlisle | 3 | 1 | Charlton | M Haywood |
| 14/11/2009 | Charlton | 5 | 1 | Milton Keynes Dons | K Hill |
| 21/11/2009 | Yeovil | 1 | 1 | Charlton | S Hooper |
| 24/11/2009 | Charlton | 4 | 2 | Bristol Rvs | S Cook |
| 01/12/2009 | Brighton | 0 | 2 | Charlton | G Ward |
| 05/12/2009 | Charlton | 1 | 0 | Southend | D McDermid |
| 12/12/2009 | Stockport | 1 | 2 | Charlton | E Ilderton |
| 19/12/2009 | Charlton | 4 | 4 | Millwall | M Jones |
| 26/12/2009 | Charlton | 2 | 2 | Swindon | P Miller |
| 28/12/2009 | Brentford | 1 | 1 | Charlton | A D'Urso |
| 16/01/2010 | Wycombe | 1 | 2 | Charlton | K Evans |
| 19/01/2010 | Charlton | 2 | 1 | Hartlepool | S Tanner |
| 25/01/2010 | Charlton | 0 | 1 | Leyton Orient | J Moss |
| 30/01/2010 | Charlton | 1 | 1 | Tranmere | G Sutton |
| 02/02/2010 | Walsall | 1 | 1 | Charlton | A Haines |
| 06/02/2010 | Swindon | 1 | 1 | Charlton | A Hall |
| 15/02/2010 | Bristol Rvs | 2 | 1 | Charlton | T Kettle |
| 20/02/2010 | Charlton | 2 | 0 | Yeovil | F Graham |
| 23/02/2010 | Charlton | 1 | 2 | Brighton | A D'Urso |
| 26/02/2010 | Southend | 1 | 2 | Charlton | K Wright |
| 06/03/2010 | Charlton | 2 | 0 | Stockport | K Stroud |
| 13/03/2010 | Millwall | 4 | 0 | Charlton | N Swarbrick |
| 20/03/2010 | Charlton | 2 | 2 | Gillingham | P Taylor |
| 27/03/2010 | Huddersfield | 1 | 1 | Charlton | J Singh |
| 03/04/2010 | Milton Keynes Dons | 0 | 1 | Charlton | A Bates |
| 05/04/2010 | Charlton | 1 | 0 | Carlisle | J Linington |
| 10/04/2010 | Southampton | 1 | 0 | Charlton | G Hegley |
| 13/04/2010 | Charlton | 1 | 0 | Colchester | M Jones |
| 17/04/2010 | Charlton | 0 | 1 | Norwich | A Hall |
| 24/04/2010 | Exeter | 1 | 1 | Charlton | D Phillips |
| 01/05/2010 | Charlton | 1 | 0 | Leeds | L Probert |
| 08/05/2010 | Oldham | 0 | 2 | Charlton | C Webster |