Key: Win : Draw : Loss
| Date | Home | Away | Ref | ||
|---|---|---|---|---|---|
| 08/08/2009 | Carlisle | 1 | 3 | Brentford | M Haywood |
| 15/08/2009 | Millwall | 0 | 0 | Carlisle | D Deadman |
| 18/08/2009 | Stockport | 1 | 2 | Carlisle | A Hall |
| 22/08/2009 | Carlisle | 0 | 1 | Exeter | M Oliver |
| 29/08/2009 | Leyton Orient | 2 | 2 | Carlisle | G Horwood |
| 05/09/2009 | Carlisle | 3 | 0 | Tranmere | C Boyeson |
| 12/09/2009 | Carlisle | 0 | 2 | Brighton | A Haines |
| 19/09/2009 | Oldham | 2 | 0 | Carlisle | P Gibbs |
| 26/09/2009 | Carlisle | 1 | 1 | Southampton | G Salisbury |
| 29/09/2009 | Leeds | 1 | 1 | Carlisle | R Booth |
| 03/10/2009 | Walsall | 2 | 2 | Carlisle | S Cook |
| 10/10/2009 | Carlisle | 0 | 1 | Norwich | D Foster |
| 17/10/2009 | Yeovil | 3 | 1 | Carlisle | F Graham |
| 24/10/2009 | Carlisle | 2 | 1 | Southend | G Eltringham |
| 31/10/2009 | Carlisle | 3 | 1 | Charlton | M Haywood |
| 14/11/2009 | Bristol Rvs | 3 | 2 | Carlisle | G Hegley |
| 21/11/2009 | Carlisle | 0 | 1 | Swindon | C Webster |
| 24/11/2009 | Milton Keynes Dons | 3 | 4 | Carlisle | G Ward |
| 01/12/2009 | Carlisle | 3 | 2 | Hartlepool | A Taylor |
| 05/12/2009 | Gillingham | 0 | 0 | Carlisle | J Singh |
| 12/12/2009 | Carlisle | 1 | 0 | Wycombe | P Quinn |
| 28/12/2009 | Tranmere | 0 | 0 | Carlisle | P Gibbs |
| 16/01/2010 | Brentford | 3 | 1 | Carlisle | R East |
| 23/01/2010 | Carlisle | 0 | 0 | Stockport | C Sarginson |
| 26/01/2010 | Exeter | 2 | 3 | Carlisle | O Langford |
| 30/01/2010 | Carlisle | 2 | 2 | Leyton Orient | C Boyeson |
| 02/02/2010 | Colchester | 2 | 1 | Carlisle | G Sutton |
| 06/02/2010 | Huddersfield | 1 | 1 | Carlisle | D Phillips |
| 13/02/2010 | Carlisle | 5 | 0 | Milton Keynes Dons | E Ilderton |
| 16/02/2010 | Carlisle | 1 | 2 | Huddersfield | K Evans |
| 20/02/2010 | Swindon | 2 | 0 | Carlisle | D Sheldrake |
| 23/02/2010 | Hartlepool | 4 | 1 | Carlisle | C Webster |
| 27/02/2010 | Carlisle | 2 | 0 | Gillingham | P Tierney |
| 06/03/2010 | Wycombe | 0 | 0 | Carlisle | P Crossley |
| 09/03/2010 | Carlisle | 1 | 3 | Millwall | G Salisbury |
| 13/03/2010 | Carlisle | 2 | 1 | Colchester | J Waugh |
| 16/03/2010 | Carlisle | 1 | 0 | Yeovil | A Bates |
| 20/03/2010 | Southend | 2 | 2 | Carlisle | A Woolmer |
| 02/04/2010 | Carlisle | 3 | 1 | Bristol Rvs | N Swarbrick |
| 05/04/2010 | Charlton | 1 | 0 | Carlisle | J Linington |
| 10/04/2010 | Brighton | 1 | 2 | Carlisle | K Wright |
| 13/04/2010 | Carlisle | 1 | 3 | Leeds | M Halsey |
| 17/04/2010 | Carlisle | 1 | 2 | Oldham | D McDermid |
| 24/04/2010 | Southampton | 3 | 2 | Carlisle | S Hooper |
| 01/05/2010 | Carlisle | 1 | 1 | Walsall | D Foster |
| 08/05/2010 | Norwich | 0 | 2 | Carlisle | A Penn |