Key: Win : Draw : Loss
| Date | Home | Away | Ref | ||
|---|---|---|---|---|---|
| 08/08/2009 | Carlisle | 1 | 3 | Brentford | M Haywood |
| 15/08/2009 | Brentford | 0 | 0 | Brighton | T Kettle |
| 18/08/2009 | Brentford | 2 | 1 | Norwich | G Scott |
| 22/08/2009 | Southampton | 1 | 1 | Brentford | D Phillips |
| 29/08/2009 | Brentford | 1 | 1 | Oldham | G Hegley |
| 05/09/2009 | Charlton | 2 | 0 | Brentford | J Linnington |
| 12/09/2009 | Huddersfield | 0 | 0 | Brentford | D Webb |
| 19/09/2009 | Brentford | 1 | 3 | Bristol Rvs | S Hooper |
| 26/09/2009 | Yeovil | 2 | 0 | Brentford | R East |
| 29/09/2009 | Brentford | 2 | 1 | Southend | K Wright |
| 03/10/2009 | Brentford | 2 | 3 | Swindon | O Langford |
| 09/10/2009 | Hartlepool | 0 | 0 | Brentford | G Salisbury |
| 17/10/2009 | Leyton Orient | 2 | 1 | Brentford | A D'Urso |
| 24/10/2009 | Brentford | 2 | 0 | Stockport | G Ward |
| 31/10/2009 | Exeter | 3 | 0 | Brentford | D Whitestone |
| 14/11/2009 | Brentford | 2 | 2 | Millwall | A Woolmer |
| 21/11/2009 | Brentford | 1 | 1 | Walsall | G Sutton |
| 24/11/2009 | Wycombe | 1 | 0 | Brentford | K Stroud |
| 01/12/2009 | Brentford | 1 | 0 | Colchester | G Scott |
| 05/12/2009 | Tranmere | 1 | 0 | Brentford | C Sarginson |
| 12/12/2009 | Brentford | 0 | 0 | Leeds | D Deadman |
| 19/12/2009 | Milton Keynes Dons | 0 | 1 | Brentford | A Penn |
| 26/12/2009 | Gillingham | 0 | 1 | Brentford | F Graham |
| 28/12/2009 | Brentford | 1 | 1 | Charlton | A D'Urso |
| 16/01/2010 | Brentford | 3 | 1 | Carlisle | R East |
| 23/01/2010 | Norwich | 1 | 0 | Brentford | S Rushton |
| 26/01/2010 | Brentford | 1 | 1 | Southampton | C Sarginson |
| 06/02/2010 | Brentford | 4 | 0 | Gillingham | P Miller |
| 13/02/2010 | Brentford | 1 | 1 | Wycombe | C Boyeson |
| 20/02/2010 | Walsall | 2 | 1 | Brentford | J Moss |
| 06/03/2010 | Leeds | 1 | 1 | Brentford | O Langford |
| 13/03/2010 | Brentford | 3 | 3 | Milton Keynes Dons | M Russell |
| 16/03/2010 | Brighton | 3 | 0 | Brentford | D McDermid |
| 20/03/2010 | Stockport | 0 | 1 | Brentford | N Miller |
| 23/03/2010 | Colchester | 3 | 3 | Brentford | F Graham |
| 27/03/2010 | Brentford | 1 | 0 | Leyton Orient | K Stroud |
| 30/03/2010 | Oldham | 2 | 3 | Brentford | A Bates |
| 02/04/2010 | Millwall | 1 | 1 | Brentford | P Taylor |
| 05/04/2010 | Brentford | 0 | 0 | Exeter | M Russell |
| 10/04/2010 | Brentford | 3 | 0 | Huddersfield | A Hall |
| 13/04/2010 | Southend | 2 | 2 | Brentford | T Kettle |
| 17/04/2010 | Bristol Rvs | 0 | 0 | Brentford | P Tierney |
| 20/04/2010 | Brentford | 2 | 1 | Tranmere | D Phillips |
| 24/04/2010 | Brentford | 1 | 1 | Yeovil | G Horwood |
| 01/05/2010 | Swindon | 3 | 2 | Brentford | A Woolmer |
| 08/05/2010 | Brentford | 0 | 0 | Hartlepool | D McDermid |